Determination of urinary urate with alkylamine glass-bound uricase.
نویسندگان
چکیده
Porcine liver uricase immobilized on alkylamine glass (pore diameter 55 nm) through glutaraldehyde coupling has been found useful in the determination of uri c acid in urine. The minimum detec.tion limit was 12.0 J.lg/0.1 ml sample and the recovery of added uric acid was 91 %. The coefficient of vari ation (CV) were <6.0% and <2.0% for within and between batch assay respectively. A good correlation (r=0.93 ) was found between urate values obtained by a standard commercial method and the present method .
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ورودعنوان ژورنال:
- Indian journal of biochemistry & biophysics
دوره 37 2 شماره
صفحات -
تاریخ انتشار 2000